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Ms. Stanislav Alexandra Alina

 

Ms. Stanislav Alexandra Alina

COUNTY EMERGENCY HOSPITAL GIURGIU
ROMANIA

Abstract Title: Association between Metabolic Syndrome and APOA5 gene rs662799 and APOE 4(E4/E4) gene in obese patients.

Biography:

Biography: Stanislav Alexandra Alina is a medical biologist specialist at the Department of Biochemistry within the Medical Analysis Laboratory of the Emergency County Hospital Giurgiu Romania and completed her PhD at the age of 35 at the Department of Genetics, Faculty of Biology, University of Bucharest. She has studies in classical and modern methods of human genetics, biochemical and hematological laboratory investigations, clinical aspects (Metabolic syndrome, obesity, diabetes mellitus and hypertension, risk factors and bioinformatics methods, epigenetics (Alzheimer's), microbiology(Staphylococcus aureus), Immunology, Biochemistry, urinary biochemistry, Electrophoresis, Ionometry and HbA1c and Matlab programming, RFLP-PCR and Advanced NGx.

Research Interest:

Introduction: The APOA5 gene rs662799 is associated with metabolic syndrome, diabetes mellitus, obesity and hypertension. The APOE 4 gene with the E4/E4 isoform is associated with insulin resistance, BMI, HTN, cognitive dysfunction, Alzheimer’s disease, cardiovascular disease, dyslipidemia, diabetes, obesity and inflammation. Purpose: Testing the association between the APOA5 gene rs662799 and the APOE 4 E4/E4 gene and metabolic syndrome based on clinical data, biochemical, hematological, genetic and bioinformatics laboratory investigations in obese patients. Materials and methods. Both genes were identified by RFLP-PCR, the Advanced NGx test, 75 MS subjects from the Giurgiu County Emergency Hospital were selected (55 obese patients and 20 controls) based on the following parameters: BMI, HTA, cholesterol, triglycerides, LDL, HDL, uric acid, glucose, HbA1c processed by the Graph Pad Prism 7.0.3 program and Matlab R2009b.Results:The results obtained through bioinformatics programs showed that the APOA5 gene with the rs662799 polymorphisms and APOE E4/E4 presents BMI, HTA, cholesterol, LDL, triglycerides, glucose and uric acid increased and low HDL are associated with obesity, diabetes, hypertension and metabolic syndrome, and results that it is associated with Alzheimer’s disease and metabolic syndrome.Conclusions: The APOA5 gene rs662799 and the APOE E4/E4 gene are associated with metabolic syndrome and Alzheimer’s disease.